Part:BBa_K5184004:Design

slCPR2

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

"Design Considerations:

1.Optimization of all codons for E. coli based on E. coli codon bias 2.Removal of N-terminus anchoring region to avoid incompatibility between the membrane recognition signal of heterogeneous proteins and the eukaryotic host. 3.Intergration of Spytag-spycathcer system, allowing the fusion of SlCPR2 and its redox partner ShZPO and thus enabling more efficient electron transfer. "

Source

Solanum lycopersicum